Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate.
Identifieur interne : 001836 ( Main/Exploration ); précédent : 001835; suivant : 001837Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate.
Auteurs : S D Ji [République populaire de Chine] ; Z Y Wang [République populaire de Chine] ; H J Fan [République populaire de Chine] ; R S Zhang [République populaire de Chine] ; Z Y Yu [République populaire de Chine] ; J J Wang [République populaire de Chine] ; Z H Liu [République populaire de Chine]Source :
- Journal of plant research [ 1618-0860 ] ; 2016.
Descripteurs français
- KwdFr :
- Alternaria (génétique), Antifongiques (métabolisme), Ascomycota (génétique), Clonage moléculaire (MeSH), Fermentation (génétique), Gènes fongiques (MeSH), Myeloperoxidase (métabolisme), Perméabilité des membranes cellulaires (MeSH), Populus (génétique), Populus (microbiologie), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes fongiques (MeSH), Stress physiologique (génétique), Superoxide dismutase (métabolisme), Séquence nucléotidique (MeSH), Transcription génétique (MeSH), Transformation génétique (MeSH), Trichoderma (génétique), Trichoderma (métabolisme), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Alternaria, Ascomycota, Fermentation, Populus, Protéines fongiques, Régions promotrices (génétique), Stress physiologique, Trichoderma.
- microbiologie : Populus.
- métabolisme : Antifongiques, Myeloperoxidase, Protéines fongiques, Superoxide dismutase, Trichoderma.
- Clonage moléculaire, Gènes fongiques, Perméabilité des membranes cellulaires, Régulation de l'expression des gènes fongiques, Séquence nucléotidique, Transcription génétique, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Alternaria (genetics), Antifungal Agents (metabolism), Ascomycota (genetics), Base Sequence (MeSH), Cell Membrane Permeability (MeSH), Cloning, Molecular (MeSH), Fermentation (genetics), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Fungal (MeSH), Genes, Fungal (MeSH), Peroxidase (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (microbiology), Promoter Regions, Genetic (genetics), Stress, Physiological (genetics), Superoxide Dismutase (metabolism), Transcription, Genetic (MeSH), Transformation, Genetic (MeSH), Trichoderma (genetics), Trichoderma (metabolism).
- MESH :
- chemical , genetics : Fungal Proteins.
- chemical , metabolism : Antifungal Agents, Fungal Proteins, Peroxidase, Superoxide Dismutase.
- genetics : Alternaria, Ascomycota, Fermentation, Populus, Promoter Regions, Genetic, Stress, Physiological, Trichoderma.
- metabolism : Trichoderma.
- microbiology : Populus.
- Base Sequence, Cell Membrane Permeability, Cloning, Molecular, Gene Expression Regulation, Fungal, Genes, Fungal, Plants, Genetically Modified, Transcription, Genetic, Transformation, Genetic.
Abstract
The tolerance of plants to biotic and abiotic stresses could be improved by transforming with fungal resistance-related genes. In this study, the cDNA sequence (GenBank Acc. No. KP337939) of the resistance-related gene Hsp24 encoding the 24 kD heat shock protein was obtained from the biocontrol fungus Trichoderma asperellum ACCC30536. The promoter region of Hsp24 contained many cis-regulators related to stresses response, such as "GCN4" and "GCR1" etc. Hsp24 transcription in T. asperellum was up-regulated under six different environmental stresses, compared with the control. Furthermore, following heterologous transformation into Populus davidiana × P. alba var. Pyramidalis (Pdpap), Hsp24 was successfully transcribed in transformant Pdpap-Hsp24s. Pathogen-related genes (PRs) in four Pdpap-Hsp24s were up-regulated compared with those in the control Pdpap (Pdpap-Con). After co-culture of Pdpap-Hsp24s with the weak parasite Cytospora chrysosperma, the transcription of genes related to hormone signal pathway (JA and SA) were up-regulated in Pdpap-Hsp24s, and ethidium bromide (EtBr) and Nitro-blue tetrazolium (NBT) staining assays indicated that the cell membrane permeability and the active oxygen content of Pdpap-Hsp24s leaves were lower than that of the control Pdpap-Con. And when the Pdpap-Hsp24s were under the Alternaria alternate stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) got higher in Pdpap-Hsp24s than that in Pdpap-Con, and the disease spots in Pdpap-Con leaves were obviously larger than those in Pdpap-Hsp24s leaves. In summary, Hsp24 of T. asperellum ACCC30536 is an important defense response gene, and its heterologous expression improved the resistance of transformant Pdpap-Hsp24s to C. chrysosperma and A. alternate.
DOI: 10.1007/s10265-016-0829-9
PubMed: 27193371
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate.</title><author><name sortKey="Ji, S D" sort="Ji, S D" uniqKey="Ji S" first="S D" last="Ji">S D Ji</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Z Y" sort="Wang, Z Y" uniqKey="Wang Z" first="Z Y" last="Wang">Z Y Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Fan, H J" sort="Fan, H J" uniqKey="Fan H" first="H J" last="Fan">H J Fan</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, R S" sort="Zhang, R S" uniqKey="Zhang R" first="R S" last="Zhang">R S Zhang</name><affiliation wicri:level="1"><nlm:affiliation>The College of Landscape, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The College of Landscape, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Z Y" sort="Yu, Z Y" uniqKey="Yu Z" first="Z Y" last="Yu">Z Y Yu</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Wang, J J" sort="Wang, J J" uniqKey="Wang J" first="J J" last="Wang">J J Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Z H" sort="Liu, Z H" uniqKey="Liu Z" first="Z H" last="Liu">Z H Liu</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China. LZHNEFU@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27193371</idno><idno type="pmid">27193371</idno><idno type="doi">10.1007/s10265-016-0829-9</idno><idno type="wicri:Area/Main/Corpus">001789</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001789</idno><idno type="wicri:Area/Main/Curation">001789</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001789</idno><idno type="wicri:Area/Main/Exploration">001789</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate.</title><author><name sortKey="Ji, S D" sort="Ji, S D" uniqKey="Ji S" first="S D" last="Ji">S D Ji</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Z Y" sort="Wang, Z Y" uniqKey="Wang Z" first="Z Y" last="Wang">Z Y Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Fan, H J" sort="Fan, H J" uniqKey="Fan H" first="H J" last="Fan">H J Fan</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, R S" sort="Zhang, R S" uniqKey="Zhang R" first="R S" last="Zhang">R S Zhang</name><affiliation wicri:level="1"><nlm:affiliation>The College of Landscape, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The College of Landscape, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Z Y" sort="Yu, Z Y" uniqKey="Yu Z" first="Z Y" last="Yu">Z Y Yu</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Wang, J J" sort="Wang, J J" uniqKey="Wang J" first="J J" last="Wang">J J Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Z H" sort="Liu, Z H" uniqKey="Liu Z" first="Z H" last="Liu">Z H Liu</name><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China. LZHNEFU@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of plant research</title><idno type="eISSN">1618-0860</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alternaria (genetics)</term><term>Antifungal Agents (metabolism)</term><term>Ascomycota (genetics)</term><term>Base Sequence (MeSH)</term><term>Cell Membrane Permeability (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Fermentation (genetics)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Peroxidase (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (microbiology)</term><term>Promoter Regions, Genetic (genetics)</term><term>Stress, Physiological (genetics)</term><term>Superoxide Dismutase (metabolism)</term><term>Transcription, Genetic (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Trichoderma (genetics)</term><term>Trichoderma (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alternaria (génétique)</term><term>Antifongiques (métabolisme)</term><term>Ascomycota (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Fermentation (génétique)</term><term>Gènes fongiques (MeSH)</term><term>Myeloperoxidase (métabolisme)</term><term>Perméabilité des membranes cellulaires (MeSH)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Stress physiologique (génétique)</term><term>Superoxide dismutase (métabolisme)</term><term>Séquence nucléotidique (MeSH)</term><term>Transcription génétique (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Trichoderma (génétique)</term><term>Trichoderma (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antifungal Agents</term><term>Fungal Proteins</term><term>Peroxidase</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Alternaria</term><term>Ascomycota</term><term>Fermentation</term><term>Populus</term><term>Promoter Regions, Genetic</term><term>Stress, Physiological</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alternaria</term><term>Ascomycota</term><term>Fermentation</term><term>Populus</term><term>Protéines fongiques</term><term>Régions promotrices (génétique)</term><term>Stress physiologique</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antifongiques</term><term>Myeloperoxidase</term><term>Protéines fongiques</term><term>Superoxide dismutase</term><term>Trichoderma</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cell Membrane Permeability</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Fungal</term><term>Genes, Fungal</term><term>Plants, Genetically Modified</term><term>Transcription, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Gènes fongiques</term><term>Perméabilité des membranes cellulaires</term><term>Régulation de l'expression des gènes fongiques</term><term>Séquence nucléotidique</term><term>Transcription génétique</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The tolerance of plants to biotic and abiotic stresses could be improved by transforming with fungal resistance-related genes. In this study, the cDNA sequence (GenBank Acc. No. KP337939) of the resistance-related gene Hsp24 encoding the 24 kD heat shock protein was obtained from the biocontrol fungus Trichoderma asperellum ACCC30536. The promoter region of Hsp24 contained many cis-regulators related to stresses response, such as "GCN4" and "GCR1" etc. Hsp24 transcription in T. asperellum was up-regulated under six different environmental stresses, compared with the control. Furthermore, following heterologous transformation into Populus davidiana × P. alba var. Pyramidalis (Pdpap), Hsp24 was successfully transcribed in transformant Pdpap-Hsp24s. Pathogen-related genes (PRs) in four Pdpap-Hsp24s were up-regulated compared with those in the control Pdpap (Pdpap-Con). After co-culture of Pdpap-Hsp24s with the weak parasite Cytospora chrysosperma, the transcription of genes related to hormone signal pathway (JA and SA) were up-regulated in Pdpap-Hsp24s, and ethidium bromide (EtBr) and Nitro-blue tetrazolium (NBT) staining assays indicated that the cell membrane permeability and the active oxygen content of Pdpap-Hsp24s leaves were lower than that of the control Pdpap-Con. And when the Pdpap-Hsp24s were under the Alternaria alternate stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) got higher in Pdpap-Hsp24s than that in Pdpap-Con, and the disease spots in Pdpap-Con leaves were obviously larger than those in Pdpap-Hsp24s leaves. In summary, Hsp24 of T. asperellum ACCC30536 is an important defense response gene, and its heterologous expression improved the resistance of transformant Pdpap-Hsp24s to C. chrysosperma and A. alternate. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27193371</PMID><DateCompleted><Year>2017</Year><Month>04</Month><Day>10</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-0860</ISSN><JournalIssue CitedMedium="Internet"><Volume>129</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of plant research</Title><ISOAbbreviation>J Plant Res</ISOAbbreviation></Journal><ArticleTitle>Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate.</ArticleTitle><Pagination><MedlinePgn>921-933</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10265-016-0829-9</ELocationID><Abstract><AbstractText>The tolerance of plants to biotic and abiotic stresses could be improved by transforming with fungal resistance-related genes. In this study, the cDNA sequence (GenBank Acc. No. KP337939) of the resistance-related gene Hsp24 encoding the 24 kD heat shock protein was obtained from the biocontrol fungus Trichoderma asperellum ACCC30536. The promoter region of Hsp24 contained many cis-regulators related to stresses response, such as "GCN4" and "GCR1" etc. Hsp24 transcription in T. asperellum was up-regulated under six different environmental stresses, compared with the control. Furthermore, following heterologous transformation into Populus davidiana × P. alba var. Pyramidalis (Pdpap), Hsp24 was successfully transcribed in transformant Pdpap-Hsp24s. Pathogen-related genes (PRs) in four Pdpap-Hsp24s were up-regulated compared with those in the control Pdpap (Pdpap-Con). After co-culture of Pdpap-Hsp24s with the weak parasite Cytospora chrysosperma, the transcription of genes related to hormone signal pathway (JA and SA) were up-regulated in Pdpap-Hsp24s, and ethidium bromide (EtBr) and Nitro-blue tetrazolium (NBT) staining assays indicated that the cell membrane permeability and the active oxygen content of Pdpap-Hsp24s leaves were lower than that of the control Pdpap-Con. And when the Pdpap-Hsp24s were under the Alternaria alternate stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) got higher in Pdpap-Hsp24s than that in Pdpap-Con, and the disease spots in Pdpap-Con leaves were obviously larger than those in Pdpap-Hsp24s leaves. In summary, Hsp24 of T. asperellum ACCC30536 is an important defense response gene, and its heterologous expression improved the resistance of transformant Pdpap-Hsp24s to C. chrysosperma and A. alternate. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>S D</ForeName><Initials>SD</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Z Y</ForeName><Initials>ZY</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>H J</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>R S</ForeName><Initials>RS</Initials><AffiliationInfo><Affiliation>The College of Landscape, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Z Y</ForeName><Initials>ZY</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>J J</ForeName><Initials>JJ</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Z H</ForeName><Initials>ZH</Initials><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, China. LZHNEFU@126.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Plant Res</MedlineTA><NlmUniqueID>9887853</NlmUniqueID><ISSNLinking>0918-9440</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.7</RegistryNumber><NameOfSubstance UI="D009195">Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000528" MajorTopicYN="N">Alternaria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002463" MajorTopicYN="N">Cell Membrane Permeability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009195" MajorTopicYN="N">Peroxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014242" MajorTopicYN="N">Trichoderma</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antifungus</Keyword><Keyword MajorTopicYN="N">Heat shock protein</Keyword><Keyword MajorTopicYN="N">Plant transgene</Keyword><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">Trichoderma asperellum</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>04</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>11</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27193371</ArticleId><ArticleId IdType="doi">10.1007/s10265-016-0829-9</ArticleId><ArticleId IdType="pii">10.1007/s10265-016-0829-9</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Appl Microbiol Biotechnol. 2007 Dec;77(4):901-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17940762</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jun;126(2):789-800</Citation><ArticleIdList><ArticleId IdType="pubmed">11402207</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2008 Nov;45(11):1506-13</Citation><ArticleIdList><ArticleId IdType="pubmed">18824239</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2011 Sep 16;9(10):749-59</Citation><ArticleIdList><ArticleId IdType="pubmed">21921934</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2007 Nov;19(11):3791-804</Citation><ArticleIdList><ArticleId IdType="pubmed">18032631</ArticleId></ArticleIdList></Reference><Reference><Citation>J Plant Physiol. 2010 May 15;167(8):659-65</Citation><ArticleIdList><ArticleId IdType="pubmed">20080316</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2012 Oct;32(10 ):1313-20</Citation><ArticleIdList><ArticleId IdType="pubmed">22971569</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2013 Dec;32(12):1953-63</Citation><ArticleIdList><ArticleId IdType="pubmed">24081610</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS J. 2014 Sep;281(17):3894-903</Citation><ArticleIdList><ArticleId IdType="pubmed">25041335</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2008;3(6):1101-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18546601</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2006 May 5;125(3):443-51</Citation><ArticleIdList><ArticleId IdType="pubmed">16678092</ArticleId></ArticleIdList></Reference><Reference><Citation>J Sci Food Agric. 2013 Dec;93(15):3691-9</Citation><ArticleIdList><ArticleId IdType="pubmed">23633231</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Biochem Biotechnol. 2014 Dec;174(8):2791-800</Citation><ArticleIdList><ArticleId IdType="pubmed">25227687</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2008 Jan;66(1-2):151-64</Citation><ArticleIdList><ArticleId IdType="pubmed">18046507</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2015 May;34(5):831-41</Citation><ArticleIdList><ArticleId IdType="pubmed">25627252</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2004 Aug;16(8):2117-27</Citation><ArticleIdList><ArticleId IdType="pubmed">15258265</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Rep. 2010 Dec;37(8):3673-81</Citation><ArticleIdList><ArticleId IdType="pubmed">20195770</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1996 Dec;112(4):1421-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8972592</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 2007 Jul;52(1):45-53</Citation><ArticleIdList><ArticleId IdType="pubmed">17581753</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2014 Oct;34(10):1118-29</Citation><ArticleIdList><ArticleId IdType="pubmed">25281841</ArticleId></ArticleIdList></Reference><Reference><Citation>Genet Mol Res. 2014 Jul 24;13(3):5350-60</Citation><ArticleIdList><ArticleId IdType="pubmed">25078591</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2013 Jun;33(6):618-27</Citation><ArticleIdList><ArticleId IdType="pubmed">23824242</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2014 Jan;98(2):533-44</Citation><ArticleIdList><ArticleId IdType="pubmed">24276619</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2015 Jun;28(6):689-700</Citation><ArticleIdList><ArticleId IdType="pubmed">25650830</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2009 Mar;149(3):1579-92</Citation><ArticleIdList><ArticleId IdType="pubmed">19176721</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2005 Feb;8(1):86-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15653405</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Sep 30;280(39):33660-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16048996</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013;8(1):e52126</Citation><ArticleIdList><ArticleId IdType="pubmed">23326325</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2014 Jan;98(2):777-83</Citation><ArticleIdList><ArticleId IdType="pubmed">24265026</ArticleId></ArticleIdList></Reference><Reference><Citation>Protoplasma. 2015 Jan;252(1):307-19</Citation><ArticleIdList><ArticleId IdType="pubmed">25064634</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Biol. 2015 May;119(5):420-32</Citation><ArticleIdList><ArticleId IdType="pubmed">25937068</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Res. 2015 Feb;171:8-20</Citation><ArticleIdList><ArticleId IdType="pubmed">25644947</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2003 Jan;15(1):63-78</Citation><ArticleIdList><ArticleId IdType="pubmed">12509522</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2003 Jun 27;113(7):935-44</Citation><ArticleIdList><ArticleId IdType="pubmed">12837250</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2009 Sep;59(6):974-86</Citation><ArticleIdList><ArticleId IdType="pubmed">19473329</ArticleId></ArticleIdList></Reference><Reference><Citation>Rice (N Y). 2015 Dec;8(1):42</Citation><ArticleIdList><ArticleId IdType="pubmed">26054241</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2006 Apr;25(4):349-58</Citation><ArticleIdList><ArticleId IdType="pubmed">16365678</ArticleId></ArticleIdList></Reference><Reference><Citation>Genet Mol Res. 2014 Feb 27;13(1):1233-43</Citation><ArticleIdList><ArticleId IdType="pubmed">24634180</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2009 Aug 24;10:393</Citation><ArticleIdList><ArticleId IdType="pubmed">19703271</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ji, S D" sort="Ji, S D" uniqKey="Ji S" first="S D" last="Ji">S D Ji</name></noRegion><name sortKey="Fan, H J" sort="Fan, H J" uniqKey="Fan H" first="H J" last="Fan">H J Fan</name><name sortKey="Liu, Z H" sort="Liu, Z H" uniqKey="Liu Z" first="Z H" last="Liu">Z H Liu</name><name sortKey="Wang, J J" sort="Wang, J J" uniqKey="Wang J" first="J J" last="Wang">J J Wang</name><name sortKey="Wang, Z Y" sort="Wang, Z Y" uniqKey="Wang Z" first="Z Y" last="Wang">Z Y Wang</name><name sortKey="Yu, Z Y" sort="Yu, Z Y" uniqKey="Yu Z" first="Z Y" last="Yu">Z Y Yu</name><name sortKey="Zhang, R S" sort="Zhang, R S" uniqKey="Zhang R" first="R S" last="Zhang">R S Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001836 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001836 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27193371
|texte= Heterologous expression of the Hsp24 from Trichoderma asperellum improves antifungal ability of Populus transformant Pdpap-Hsp24 s to Cytospora chrysosperma and Alternaria alternate.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27193371" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |